Hotspot

chlN-ycf1

SSC · 115366-116195 · 830 bp

Back to Dicranopteris

Pi 0.463

MarkerSeek score 76.90

Primer available yes

Label chlN-ycf1

Feature Metadata

label_name: chlN-ycf1
feature_type: igs
region: SSC
length_bp: 830
variable_sites: 274
pi: 0.463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.71
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN-ycf1_p1	AGCCCGATCTAGCCATTACT	GTCTCCGTACGGGACTATCA	50.0	55.0	58.0	58.0	1313 bp	0.5	59.5
chlN-ycf1_p2	AGCCCGATCTAGCCATTACT	TCTCCGTACGGGACTATCAC	50.0	55.0	58.0	58.0	1312 bp	0.5	59.5
chlN-ycf1_p3	AGCCCGATCTAGCCATTACT	TGTCTCCGTACGGGACTATC	50.0	55.0	58.0	58.0	1314 bp	0.5	59.5
chlN-ycf1_p4	AGCCCGATCTAGCCATTACT	TGTGTTGGTCGAACAAGGAG	50.0	50.0	58.0	58.1	1247 bp	0.5	59.4
chlN-ycf1_p5	AGCGTATGCATGAACCAGAG	TCTCCGTACGGGACTATCAC	50.0	55.0	58.1	58.0	1330 bp	0.5	59.2