Hotspot

ndhF

SSC · 108129-110423 · 2295 bp

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Pi 0.000915

MarkerSeek score 50.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2295
variable_sites: 5
pi: 0.000915
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGAGAAATTCTATGGCTCGGA	ACCAACTGTCACTTCTTGGT	42.9	45.0	56.5	56.9	2447-2452 bp	1.0	51.8
ndhF_p2	TGAGAAATTCTATGGCTCGGA	ACCAACTGTCACTTCTTGGTT	42.9	42.9	56.5	57.6	2447-2452 bp	1.0	49.7
ndhF_p3	TTGAGAAATTCTATGGCTCGGA	ACCAACTGTCACTTCTTGGT	40.9	45.0	57.2	56.9	2448-2453 bp	1.0	49.5
ndhF_p4	TGAGAAATTCTATGGCTCGGAT	ACCAACTGTCACTTCTTGGT	40.9	45.0	57.0	56.9	2447-2452 bp	1.0	47.8
ndhF_p5	TTGAGAAATTCTATGGCTCGGA	ACCAACTGTCACTTCTTGGTT	40.9	42.9	57.2	57.6	2448-2453 bp	1.0	47.4