Hotspot

atpF

LSC · 11074-12337 · 1264 bp

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Pi 0

MarkerSeek score 51.50

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1264
variable_sites: 0
pi: 0
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GGCTCGAATGGTTACCATGA	GAGTAAAGAAGGGGGAAAGTGA	50.0	45.5	57.7	57.4	1435-1437 bp	1.0	56.5
atpF_p2	GGCTCGAATGGTTACCATGA	AGAGTAAAGAAGGGGGAAAGTG	50.0	45.5	57.7	57.4	1436-1438 bp	1.0	56.4
atpF_p3	GGCTCGAATGGTTACCATGA	AGTAAAGAAGGGGGAAAGTGA	50.0	42.9	57.7	56.2	1434-1436 bp	1.0	54.9
atpF_p4	ACTAATTTCGTCGGCTCGAA	GAGTAAAGAAGGGGGAAAGTGA	45.0	45.5	57.4	57.4	1447-1449 bp	1.0	53.9
atpF_p5	ACTAATTTCGTCGGCTCGAA	AGAGTAAAGAAGGGGGAAAGTG	45.0	45.5	57.4	57.4	1448-1450 bp	1.0	53.9