Hotspot

accD-psaI

LSC · 57798-58502 · 705 bp

Back to Dicliptera

Pi 0.00113

MarkerSeek score 48.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 2
pi: 0.00113
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAAGCGTTCTGGGTGAGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	836 bp	1.0	79.6
accD-psaI_p2	AAAAGCGTTCTGGGTGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	835 bp	1.0	79.6
accD-psaI_p3	AAAAAGCGTTCTGGGTGAGT	GGCACAAGAATAGGGGGAAG	45.0	55.0	57.9	57.9	801 bp	1.0	79.3
accD-psaI_p4	AAAAGCGTTCTGGGTGAGTT	GGCACAAGAATAGGGGGAAG	45.0	55.0	57.9	57.9	800 bp	1.0	79.3
accD-psaI_p5	AAAAGCGTTCTGGGTGAGTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.9	57.9	841 bp	1.0	78.9