Hotspot

rbcL-accD

LSC · 55580-56324 · 745 bp

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Pi 0.00161

MarkerSeek score 48.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 3
pi: 0.00161
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	AGGAACAAGATTGTCGGTTGA	55.0	42.9	58.0	57.5	943-951 bp	1.0	68.1
rbcL-accD_p2	CCGCTTGTGAGGTATGGAAA	AGGAACAAGATTGTCGGTTGA	50.0	42.9	57.9	57.5	912-920 bp	1.0	67.5
rbcL-accD_p3	AATGGAGTCCTGAGCTAGCT	AGGAACAAGATTGTCGGTTGA	50.0	42.9	57.8	57.5	933-941 bp	1.0	67.0
rbcL-accD_p4	GGCTAGCAAATGGAGTCCTG	AGGAACAAGATTGTCGGTTGA	55.0	42.9	58.3	57.5	941-949 bp	1.0	65.8
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	CCATCGAATTAAACCACCACC	55.0	47.6	58.0	57.2	881-889 bp	1.0	65.7