Hotspot

trnT-GGU-psbD

LSC · 30654-31819 · 1166 bp

Back to Dicliptera

Pi 0.000343

MarkerSeek score 48.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1166
variable_sites: 1
pi: 0.000343
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1293-1327 bp	1.0	77.5
trnT-GGU-psbD_p2	CCCCTTTAACTCAGCGGTAG	GGACCAGCCTACAAAAACGA	55.0	50.0	57.7	58.1	1329-1363 bp	1.0	77.3
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	AAGAGTAATAGGCCGGACCA	50.0	50.0	58.3	57.8	1307-1341 bp	1.0	77.0
trnT-GGU-psbD_p4	CCCCTTTAACTCAGCGGTAG	AAGAGTAATAGGCCGGACCA	55.0	50.0	57.7	57.8	1343-1377 bp	1.0	76.8
trnT-GGU-psbD_p5	TAAGCCCCTTTAACTCAGCG	GGACCAGCCTACAAAAACGA	50.0	50.0	57.6	58.1	1333-1367 bp	1.0	76.6