Hotspot

trnE-UUC-trnT-GGU

LSC · 29974-30581 · 608 bp

Back to Dicliptera

Pi 0.00263

MarkerSeek score 50.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 608
variable_sites: 3
pi: 0.00263
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	737-777 bp	1.0	80.3
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	700-740 bp	1.0	80.3
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	734-774 bp	1.0	79.1
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	697-737 bp	1.0	79.1
trnE-UUC-trnT-GGU_p5	TTTCGTAGTACCCTACCCCC	TTGAACCGATGACTTACGCC	55.0	50.0	57.8	58.3	739-779 bp	1.0	78.1