Hotspot

petN-psbM

LSC · 27992-28964 · 973 bp

Back to Dicliptera

Pi 0.00781

MarkerSeek score 57.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 973
variable_sites: 19
pi: 0.00781
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	AGTTCCTACTGCCTTTCTACT	50.0	42.9	58.0	55.7	1062 bp	1.0	55.1
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	AGTTCCTACTGCCTTTCTACT	50.0	42.9	58.0	55.7	1060 bp	1.0	55.1
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	AGTTCCTACTGCCTTTCTACT	55.0	42.9	57.9	55.7	1059 bp	1.0	54.5
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACTGCCTTTCTACT	50.0	42.9	58.2	55.7	1114 bp	1.0	53.8
petN-psbM_p5	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACTGCCT	50.0	42.9	58.0	55.5	1067 bp	1.0	53.4