Hotspot

rpoB-trnC-GCA

LSC · 26086-27298 · 1213 bp

Back to Dicliptera

Pi 0.00066

MarkerSeek score 51.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1213
variable_sites: 2
pi: 0.00066
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGTTCTTCTGTCAAACCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1378-1393 bp	1.0	77.5
rpoB-trnC-GCA_p2	GTCAAACCCCGGTCAATGAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1369-1384 bp	1.0	76.4
rpoB-trnC-GCA_p3	TCAAACCCCGGTCAATGAAC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1368-1383 bp	1.0	76.4
rpoB-trnC-GCA_p4	CCCGGTCAATGAACCTACAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1362-1377 bp	1.0	75.7
rpoB-trnC-GCA_p5	AGTTCTTCTGTCAAACCCCG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	58.4	1360-1375 bp	1.0	75.3