Hotspot

ycf1

SSC · 122747-128455 · 5709 bp

Back to Dichocarpum

Pi 0.0248

MarkerSeek score 53.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5709
variable_sites: 210
pi: 0.0248
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTCCGCTGCTATGTTGTTCT	CTCTCTTCCTTGTCACAGGC	50.0	55.0	58.0	57.9	1299-1311 bp	1.0	84.0
ycf1_p2	GTTCATCGGATCCAAGACCC	CTCTCTTCCTTGTCACAGGC	55.0	55.0	58.0	57.9	1174-1186 bp	1.0	83.4
ycf1_p3	GGTATGATCCGCGGTTGAAT	CTCTCTTCCTTGTCACAGGC	50.0	55.0	58.1	57.9	992-1001 bp	1.0	82.8
ycf1_p4	TGTTTGATTGGCGGGGTTTA	CTCTCTTCCTTGTCACAGGC	45.0	55.0	58.0	57.9	861 bp	0.666667	67.8
ycf1_p5	TGTTTGATTGGCGGGGTTTA	TTTATGGGCTGGCGTTACAA	45.0	45.0	58.0	57.8	350 bp	0.666667	67.8