Hotspot

ndhF

SSC · 109791-111974 · 2184 bp

Back to Dichocarpum

Pi 0.0249

MarkerSeek score 58.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2184
variable_sites: 81
pi: 0.0249
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.0	57.9	2459-2471 bp	1.0	81.7
ndhF_p2	AAGAGGGATCCACCGAAGAA	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.0	57.9	2451-2463 bp	1.0	81.7
ndhF_p3	CCTTCCCCTTCCCTTTGTTC	AGAGCTGGTGAATCGGAAAC	55.0	50.0	58.1	57.9	2428-2440 bp	1.0	81.5
ndhF_p4	GGGGACTGAACAGGAACAAG	AGAGCTGGTGAATCGGAAAC	55.0	50.0	58.1	57.9	2468-2480 bp	1.0	81.3
ndhF_p5	AGGGGGAAAAGTGAGGAAGA	AGAGCTGGTGAATCGGAAAC	50.0	50.0	57.9	57.9	2524-2536 bp	1.0	80.9