Hotspot

ndhD

SSC · 114414-115574 · 1161 bp

Back to Dichocarpum

Pi 0.00976

MarkerSeek score 50.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1161
variable_sites: 17
pi: 0.00976
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCTATGCAGCTCTTCTGTGT	GCATGAGACAACTCGTAGCA	50.0	50.0	58.0	58.0	2410-2431 bp	1.0	82.7
ndhD_p2	GCAAGGCTCAAATTCTGCAG	GCATGAGACAACTCGTAGCA	50.0	50.0	58.0	58.0	1989-2016 bp	1.0	82.6
ndhD_p3	GCTATGCAGCTCTTCTGTGT	CGGATTTCCTAAGTGTCCGG	50.0	55.0	58.0	58.1	2441-2462 bp	1.0	82.2
ndhD_p4	GCAAGGCTCAAATTCTGCAG	CGGATTTCCTAAGTGTCCGG	50.0	55.0	58.0	58.1	2020-2047 bp	1.0	82.1
ndhD_p5	GCAAGGCTCAAATTCTGCAG	GAGACAACTCGTAGCATGGG	50.0	55.0	58.0	58.1	1985-2012 bp	1.0	82.1