Hotspot

infA

LSC · 79086-79214 · 129 bp

Back to Dichocarpum

Pi 0.0517

MarkerSeek score 69.60

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 129
variable_sites: 9
pi: 0.0517
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	CCACCCGACCCTGTATATCT	AAAACGTATCAGTCGACCCG	55.0	50.0	58.0	58.0	1022-1047 bp	1.0	84.7
infA_p2	CCACCCGACCCTGTATATCT	TTGGTTTCAACTCTGCGACA	55.0	45.0	58.0	58.0	1078-1103 bp	1.0	84.3
infA_p3	GCATCTCTTCCAAGACCAGG	AAAACGTATCAGTCGACCCG	55.0	50.0	58.0	58.0	1182-1207 bp	1.0	84.0
infA_p4	AAATTCTGCGTTCCCTGGTT	AAAACGTATCAGTCGACCCG	45.0	50.0	58.0	58.0	509-510 bp	1.0	83.8
infA_p5	AAATTCTGCGTTCCCTGGTT	TTGGTTTCAACTCTGCGACA	45.0	45.0	58.0	58.0	565-566 bp	1.0	83.2