Hotspot

rps15-ycf1

SSC · 122344-122746 · 403 bp

Back to Dichocarpum

Pi 0.0377

MarkerSeek score 68.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 403
variable_sites: 22
pi: 0.0377
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CCGCAGACCTCTCTGAGATA	ACCCTGTATTTGGCAATGGG	55.0	50.0	58.0	58.1	780-786 bp	0.666667	68.2
rps15-ycf1_p2	CCGCAGACCTCTCTGAGATA	CCCTGTATTTGGCAATGGGA	55.0	50.0	58.0	57.8	779-785 bp	0.666667	68.1
rps15-ycf1_p3	CCAATATGGGTTCGCAGTCA	ACCCTGTATTTGGCAATGGG	50.0	50.0	58.0	58.1	1157-1163 bp	0.666667	67.7
rps15-ycf1_p4	AACACCATGCATTGATGGGT	ACCCTGTATTTGGCAATGGG	45.0	50.0	58.1	58.1	1099-1105 bp	0.666667	67.7
rps15-ycf1_p5	ACCATGCATTGATGGGTGTT	ACCCTGTATTTGGCAATGGG	45.0	50.0	58.1	58.1	1096-1102 bp	0.666667	67.7