Hotspot

ndhD-psaC

SSC · 115575-116056 · 482 bp

Back to Dichocarpum

Pi 0.0363

MarkerSeek score 66.60

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 482
variable_sites: 26
pi: 0.0363
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TTTGACCGCTGTACATTGCT	GCATGAGACAACTCGTAGCA	45.0	50.0	58.1	58.0	538-544 bp	1.0	84.1
ndhD-psaC_p2	CCCCTGCGGTGTACAAAATA	GCATGAGACAACTCGTAGCA	50.0	50.0	57.9	58.0	682-688 bp	1.0	83.0
ndhD-psaC_p3	CTGACCCGGAGATGTTGAAG	GCATGAGACAACTCGTAGCA	55.0	50.0	58.0	58.0	1056-1062 bp	1.0	82.7
ndhD-psaC_p4	CTGACCCGGAGATGTTGAAG	CGGATTTCCTAAGTGTCCGG	55.0	55.0	58.0	58.1	1087-1093 bp	1.0	82.2
ndhD-psaC_p5	CTGACCCGGAGATGTTGAAG	GAGACAACTCGTAGCATGGG	55.0	55.0	58.0	58.1	1052-1058 bp	1.0	82.2