Hotspot

ccsA-ndhD

SSC · 114232-114413 · 182 bp

Back to Dichocarpum

Pi 0.0659

MarkerSeek score 72.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 182
variable_sites: 17
pi: 0.0659
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCTATGCAGCTCTTCTGTGT	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.0	58.0	1102-1129 bp	1.0	83.1
ccsA-ndhD_p2	GCAAGGCTCAAATTCTGCAG	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.0	58.0	681-714 bp	1.0	83.0
ccsA-ndhD_p3	GCAAGGCTCAAATTCTGCAG	CTCGATGGCTTCTCTTGCAT	50.0	50.0	58.0	58.0	679-712 bp	1.0	82.8
ccsA-ndhD_p4	GCTATGCAGCTCTTCTGTGT	CTTCAACATCTCCGGGTCAG	50.0	55.0	58.0	58.0	1368-1395 bp	1.0	82.6
ccsA-ndhD_p5	GCAAGGCTCAAATTCTGCAG	CTTCAACATCTCCGGGTCAG	50.0	55.0	58.0	58.0	947-980 bp	1.0	82.3