Hotspot

ndhF-trnL-UAG

SSC · 111975-113056 · 1082 bp

Back to Dichocarpum

Pi 0.0553

MarkerSeek score 86.20

Primer available yes

Label ndhF-trnL-UAG

Feature Metadata

label_name: ndhF-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1082
variable_sites: 85
pi: 0.0553
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL-UAG_p1	AGGAACTGGAAGGGGAACTA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.3	58.0	1146-1174 bp	1.0	83.8
ndhF-trnL-UAG_p2	TAGGAACTGGAAGGGGAACT	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.3	58.0	1147-1175 bp	1.0	83.8
ndhF-trnL-UAG_p3	AGGAACTGGAAGGGGAACTA	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.3	58.0	1147-1175 bp	1.0	83.8
ndhF-trnL-UAG_p4	TAGGAACTGGAAGGGGAACT	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.3	58.0	1148-1176 bp	1.0	83.8
ndhF-trnL-UAG_p5	AGGAACTGGAAGGGGAACTA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.3	57.9	1156-1184 bp	1.0	82.8