Hotspot

rpl14-rpl16

LSC · 80403-80539 · 137 bp

Back to Dichocarpum

Pi 0.0245

MarkerSeek score 70.40

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 5
pi: 0.0245
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ATCGGCGATTACTAGCTCCT	GCGTCCAAAATGCCTATACG	50.0	50.0	58.1	57.3	276-280 bp	1.0	76.3
rpl14-rpl16_p2	ACATTAATTCTCTCGCCCCG	GCGTCCAAAATGCCTATACG	50.0	50.0	57.8	57.3	243-247 bp	1.0	75.4
rpl14-rpl16_p3	TCGGCGATTACTAGCTCCTA	GCGTCCAAAATGCCTATACG	50.0	50.0	57.4	57.3	275-279 bp	1.0	72.6
rpl14-rpl16_p4	ATCGGCGATTACTAGCTCCT	CTATAGCTGCGTCCAAAATGC	50.0	47.6	58.1	57.7	284-288 bp	1.0	71.5
rpl14-rpl16_p5	ATCGGCGATTACTAGCTCCT	GCTGCGTCCAAAATGCCTAT	50.0	50.0	58.1	59.3	279-283 bp	1.0	71.5