Hotspot

psbE-petL

LSC · 64062-65066 · 1005 bp

Back to Dichocarpum

Pi 0.0285

MarkerSeek score 67.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1005
variable_sites: 42
pi: 0.0285
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ACGGTAGGTACAGCTAGTCC	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.0	58.0	1918-2002 bp	1.0	84.5
psbE-petL_p2	GGTAGGTACAGCTAGTCCGT	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.0	58.0	1916-2000 bp	1.0	84.5
psbE-petL_p3	TCGTTCGGGTTTGATTGTGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	2009-2093 bp	1.0	84.4
psbE-petL_p4	CAAGTGGTCGTGCTAGAGAC	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.0	58.0	2196-2280 bp	1.0	84.2
psbE-petL_p5	CAAGTGGTCGTGCTAGAGAC	GGTAGGTAAAAGGGGGAGGT	55.0	55.0	58.0	58.0	1873-1926 bp	0.666667	68.5