Pi
0.0267
MarkerSeek score
70.30
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 554-824 bp | 1.0 | 85.8 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 562-832 bp | 1.0 | 85.2 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | AACCCAATCAAGTCCGTAGC | 55.0 | 50.0 | 57.9 | 57.9 | 578-848 bp | 1.0 | 84.8 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 586-856 bp | 1.0 | 84.8 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 594-864 bp | 1.0 | 84.2 |