Hotspot

trnE-UUC-trnT-GGU

LSC · 30991-31680 · 690 bp

Back to Dichocarpum

Pi 0.0324

MarkerSeek score 73.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 31
pi: 0.0324
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	831-862 bp	1.0	83.2
trnE-UUC-trnT-GGU_p2	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	793-824 bp	1.0	82.6
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	828-859 bp	1.0	82.1
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	TGGAAAAAGCCCCTTATCGG	55.0	50.0	57.8	57.9	855-886 bp	1.0	82.0
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	TGGAAAAAGCCCCTTATCGG	55.0	50.0	58.3	57.9	817-848 bp	1.0	81.4