Hotspot

petN-psbM

LSC · 28006-29169 · 1164 bp

Back to Dichocarpum

Pi 0.0283

MarkerSeek score 70.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1164
variable_sites: 47
pi: 0.0283
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.0	57.6	1306-1326 bp	1.0	72.9
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	1312-1332 bp	1.0	71.4
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1252-1272 bp	1.0	70.3
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTC	45.0	45.0	58.0	56.1	1306-1326 bp	1.0	68.7
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTC	50.0	45.0	57.8	56.1	1312-1332 bp	1.0	67.2