Hotspot

rps16-trnQ-UUG

LSC · 5800-6265 · 466 bp

Back to Dichocarpum

Pi 0.0378
MarkerSeek score 72.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
466
variable_sites
26
pi
0.0378
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 535-759 bp 1.0 83.3
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 539-763 bp 1.0 82.1
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA ACTCGGAGGTTCGAATCCTT 45.0 50.0 58.1 58.4 540-764 bp 1.0 80.3
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA AGAAATCTAAGTGGGGCGTG 45.0 50.0 58.1 57.6 594-818 bp 1.0 80.2
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GAAATCTAAGTGGGGCGTGG 45.0 55.0 58.1 58.6 593-817 bp 1.0 78.5