Hotspot

ndhF

SSC · 101753-103969 · 2217 bp

Back to Dichanthium

Pi 0.00421

MarkerSeek score 46.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 14
pi: 0.00421
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGCATTTTGATGAGCCTCGT	AGGTTTGGTGAATCGGAAACA	45.0	42.9	58.2	58.1	2347 bp	0.333333	43.4
ndhF_p2	GTTTGCTGGCTTATTTGGCA	AGGTTTGGTGAATCGGAAACA	45.0	42.9	57.5	58.1	2486 bp	0.333333	41.2
ndhF_p3	ACGGAGACTTGCTTCACATT	AGGTTTGGTGAATCGGAAACA	45.0	42.9	57.4	58.1	2572 bp	0.333333	40.4
ndhF_p4	TGCATTTTGATGAGCCTCGT	GGTTTGGTGAATCGGAAACA	45.0	45.0	58.2	56.5	2346 bp	0.333333	40.3
ndhF_p5	CGTTTGCTGGCTTATTTGGC	AGGTTTGGTGAATCGGAAACA	50.0	42.9	58.7	58.1	2487 bp	0.333333	39.6