Hotspot

rpl32-trnL-TAG

SSC · 104979-105515 · 537 bp

Back to Dichanthium

Pi 0.0415

MarkerSeek score 72.50

Primer available yes

Label rpl32-trnL-TAG

Feature Metadata

label_name: rpl32-trnL-TAG
feature_type: igs
region: SSC
length_bp: 537
variable_sites: 33
pi: 0.0415
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-TAG_p1	GGTTTTTCTGGGCAACAAGC	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.4	58.0	633-636 bp	1.0	81.7
rpl32-trnL-TAG_p2	GGTTTTTCTGGGCAACAAGC	GGATTCGAACCGAGATGCTT	50.0	50.0	58.4	58.1	636-639 bp	1.0	81.2
rpl32-trnL-TAG_p3	GGTTTTTCTGGGCAACAAGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.4	57.9	606-609 bp	1.0	80.9
rpl32-trnL-TAG_p4	GGTTTTTCTGGGCAACAAGC	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.4	57.8	599-602 bp	1.0	80.5
rpl32-trnL-TAG_p5	GGTTTTTCTGGGCAACAAGC	GCTTGAGCACTGCTTCCTAA	50.0	50.0	58.4	58.2	620-623 bp	1.0	80.3