Hotspot

ndhF-rpl32

SSC · 103970-104798 · 829 bp

Back to Dichanthium

Pi 0.00644

MarkerSeek score 63.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 829
variable_sites: 8
pi: 0.00644
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGGAGAAGAGGA	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	914-941 bp	1.0	42.2
ndhF-rpl32_p2	CTGGAAGTGGGAGAAGAGGA	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	915-942 bp	1.0	42.2
ndhF-rpl32_p3	CTGGAAGTGGGAGAAGAGGA	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	916-943 bp	1.0	42.2
ndhF-rpl32_p4	CTGGAAGTGGGAGAAGAGGA	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	917-944 bp	1.0	42.2
ndhF-rpl32_p5	CTGGAAGTGGGAGAAGAGGA	TTTTTGACATCGAAGTACGT	55.0	35.0	58.1	53.2	918-945 bp	1.0	42.2