Hotspot

petG-trnW-CCA

LSC · 62621-62743 · 123 bp

Back to Dichanthium

Pi 0.0504

MarkerSeek score 81.70

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 123
variable_sites: 9
pi: 0.0504
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	GGGATTATTCGTGACTGCGT	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	216-220 bp	1.0	83.6
petG-trnW-CCA_p2	GCGTATTTGCAATACAGGCG	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.3	57.9	200-204 bp	1.0	82.4
petG-trnW-CCA_p3	AGCGGGATTATTCGTGACTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	219-223 bp	1.0	82.2
petG-trnW-CCA_p4	GGGATTATTCGTGACTGCGT	CCCGATGTCGTAGGTTCAAA	50.0	50.0	58.1	57.6	211-215 bp	1.0	81.4
petG-trnW-CCA_p5	GGGATTATTCGTGACTGCGT	TCCAAAACCCGATGTCGTAG	50.0	50.0	58.1	57.6	218-222 bp	1.0	81.2