Hotspot

petL-petG

LSC · 62332-62506 · 175 bp

Back to Dichanthium

Pi 0.00798

MarkerSeek score 58.00

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 175
variable_sites: 2
pi: 0.00798
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	CGGTTTTCTACTGGCTGCTT	ACGCAGTCACGAATAATCCC	50.0	50.0	58.5	58.1	316-324 bp	1.0	78.2
petL-petG_p2	TTCGGTTTTCTACTGGCTGC	ACGCAGTCACGAATAATCCC	50.0	50.0	58.5	58.1	318-326 bp	1.0	78.1
petL-petG_p3	CGGTTTTCTACTGGCTGCTT	CAGTCACGAATAATCCCGCT	50.0	50.0	58.5	57.8	313-321 bp	1.0	76.9
petL-petG_p4	TTCGGTTTTCTACTGGCTGC	CAGTCACGAATAATCCCGCT	50.0	50.0	58.5	57.8	315-323 bp	1.0	76.9
petL-petG_p5	CGGTTTTCTACTGGCTGCTT	CGCCTGTATTGCAAATACGC	50.0	50.0	58.5	58.3	332-340 bp	1.0	76.5