Hotspot

psaA-ycf3

LSC · 40717-41308 · 592 bp

Back to Dichanthium

Pi 0.00592

MarkerSeek score 66.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 5
pi: 0.00592
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GGGGCCCTTAGCTATTGTTC	TGGTTTGATCAAGCTGCTGA	55.0	45.0	58.0	57.7	811-817 bp	1.0	81.1
psaA-ycf3_p2	GTCTGGCCCATTCCTCAAAA	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.1	57.7	776-782 bp	1.0	80.8
psaA-ycf3_p3	GGGGCCCTTAGCTATTGTTC	GCTATAGCGCTTACTCCAGG	55.0	55.0	58.0	57.6	778-784 bp	1.0	80.6
psaA-ycf3_p4	GTCTGGCCCATTCCTCAAAA	GCTATAGCGCTTACTCCAGG	50.0	55.0	58.1	57.6	743-749 bp	1.0	80.3
psaA-ycf3_p5	GGTTCCGACGGACGAATAAT	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.8	57.7	714-720 bp	1.0	80.0