Hotspot

trnfM-CAT-trnT-GGT

LSC · 12652-14207 · 1556 bp

Back to Dichanthium

Pi 0.00962
MarkerSeek score 59.20
Primer available yes
Label trnfM-CAT-trnT-GGT

Feature Metadata

label_name
trnfM-CAT-trnT-GGT
feature_type
igs
region
LSC
length_bp
1556
variable_sites
22
pi
0.00962
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAT-trnT-GGT_p1 CCTCAAGGTTATGAGCCTCG ACCACTAAGTGAAAAGCCCT 55.0 45.0 57.8 56.7 1689-1749 bp 1.0 70.5
trnfM-CAT-trnT-GGT_p2 CCTCAAGGTTATGAGCCTCG TGAACCGATGACTTATGCCT 55.0 45.0 57.8 56.6 1659-1719 bp 1.0 70.4
trnfM-CAT-trnT-GGT_p3 CCTCGTGAGCTACCAAACTG ACCACTAAGTGAAAAGCCCT 55.0 45.0 58.3 56.7 1674-1734 bp 1.0 70.1
trnfM-CAT-trnT-GGT_p4 CCTCGTGAGCTACCAAACTG TGAACCGATGACTTATGCCT 55.0 45.0 58.3 56.6 1644-1704 bp 1.0 70.0
trnfM-CAT-trnT-GGT_p5 CCTCAAGGTTATGAGCCTCG CCGATGACTTATGCCTTACCA 55.0 47.6 57.8 57.5 1655-1715 bp 1.0 69.6