Hotspot

psbA-matK

LSC · 1151-1666 · 516 bp

Back to Dichanthium

Pi 0.00129

MarkerSeek score 63.00

Primer available yes

Label psbA-matK

Feature Metadata

label_name: psbA-matK
feature_type: igs
region: LSC
length_bp: 516
variable_sites: 1
pi: 0.00129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-matK_p1	AAGACGGTTTTCGGTGCTAG	CCGTATCAATGACCTGGTGA	50.0	50.0	58.2	57.0	589-662 bp	1.0	74.6
psbA-matK_p2	AAGACGGTTTTCGGTGCTAG	TCCGTATCAATGACCTGGTG	50.0	50.0	58.2	57.0	590-663 bp	1.0	74.6
psbA-matK_p3	AAAGACGGTTTTCGGTGCTA	CCGTATCAATGACCTGGTGA	45.0	50.0	57.8	57.0	590-663 bp	1.0	74.3
psbA-matK_p4	AAAGACGGTTTTCGGTGCTA	TCCGTATCAATGACCTGGTG	45.0	50.0	57.8	57.0	591-664 bp	1.0	74.3
psbA-matK_p5	TAAAGACGGTTTTCGGTGCT	CCGTATCAATGACCTGGTGA	45.0	50.0	57.8	57.0	591-664 bp	1.0	74.3