Hotspot

rbcL

LSC · 56471-57901 · 1431 bp

Back to Dichanthelium

Pi 0.00419

MarkerSeek score 45.80

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1431
variable_sites: 14
pi: 0.00419
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	AGTAGACCCTGTCGTTGTGA	TCTCTTTCTATTTTGCTTCTTTT	50.0	26.1	58.0	52.6	1548 bp	1.0	40.7
rbcL_p2	AGTAGACCCTGTCGTTGTGA	TTCTCTTTCTATTTTGCTTCTTT	50.0	26.1	58.0	52.6	1549 bp	1.0	40.7
rbcL_p3	AGTAGACCCTGTCGTTGTGA	TTTCTCTTTCTATTTTGCTTCTT	50.0	26.1	58.0	52.6	1550 bp	1.0	40.7
rbcL_p4	AGTAGACCCTGTCGTTGTGA	TTTTCTCTTTCTATTTTGCTTCT	50.0	26.1	58.0	52.6	1551 bp	1.0	40.7
rbcL_p5	AGTAGACCCTGTCGTTGTGA	AGACCTTCTTTCTATCTAGTTTT	50.0	30.4	58.0	52.5	1514 bp	1.0	40.7