Hotspot

rpl32-trnL-UAG

SSC · 107994-108526 · 533 bp

Back to Dichanthelium

Pi 0.00913
MarkerSeek score 46.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
533
variable_sites
14
pi
0.00913
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CCAAAAGGTTTTTCTGGGCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.0 57.9 608-613 bp 1.0 72.9
rpl32-trnL-UAG_p2 ACCAAAAGGTTTTTCTGGGC TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.0 57.9 609-614 bp 1.0 72.8
rpl32-trnL-UAG_p3 GGTTTTTCTGGGCAACAAACA TCCTAAGAGCAGCGTGTCTA 42.9 50.0 58.0 57.9 602-607 bp 1.0 72.7
rpl32-trnL-UAG_p4 CCAAAAGGTTTTTCTGGGCA AGCAGCGTGTCTACCAATTT 45.0 45.0 57.0 57.8 601-606 bp 1.0 72.4
rpl32-trnL-UAG_p5 CCAAAAGGTTTTTCTGGGCA GCTTGAGCACTGCTTCCTAA 45.0 50.0 57.0 58.2 622-627 bp 1.0 72.3