Hotspot

petA-psbJ

LSC · 62184-63081 · 898 bp

Back to Dichanthelium

Pi 0.00794

MarkerSeek score 48.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 898
variable_sites: 16
pi: 0.00794
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	ACGGTTCGTATTCTGGATTGG	50.0	47.6	57.9	58.1	1035-1039 bp	1.0	72.0
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	GGTTCGTATTCTGGATTGGGT	50.0	47.6	57.9	57.7	1033-1037 bp	1.0	70.6
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	CGGTTCGTATTCTGGATTGG	50.0	50.0	57.9	56.3	1034-1038 bp	1.0	67.4
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	ACGGTTCGTATTCTGGATTGG	50.0	47.6	58.7	58.1	1042-1046 bp	1.0	67.0
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	CGGTTCGTATTCTGGATTGGG	50.0	52.4	57.9	58.8	1034-1038 bp	1.0	66.5