Hotspot

trnF-GAA-ndhJ

LSC · 49564-50126 · 563 bp

Back to Dichanthelium

Pi 0.00935

MarkerSeek score 57.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 563
variable_sites: 14
pi: 0.00935
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	668-674 bp	1.0	80.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	658-664 bp	1.0	80.3
trnF-GAA-ndhJ_p3	AATCCTCGTGTCACCAGTTC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.5	57.9	671-677 bp	1.0	77.6
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGCTGGCC	50.0	55.0	58.1	58.6	667-673 bp	1.0	77.0
trnF-GAA-ndhJ_p5	AATCCTCGTGTCACCAGTTC	ATAGGCTGGCCCTTACGTAA	50.0	50.0	57.5	58.2	661-667 bp	1.0	77.0