Hotspot

trnG-GCC-trnfM-CAU

LSC · 12678-13014 · 337 bp

Back to Dichanthelium

Pi 0.00321

MarkerSeek score 51.40

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 337
variable_sites: 2
pi: 0.00321
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	CGTAGCGAGCGTAGTTCAAT	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.2	57.8	443-479 bp	1.0	81.1
trnG-GCC-trnfM-CAU_p2	ATATAGGACGTAGCGAGCGT	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.2	57.8	451-487 bp	1.0	80.9
trnG-GCC-trnfM-CAU_p3	CGTAGCGAGCGTAGTTCAAT	CAGTTTGGTAGCTCACGAGG	50.0	55.0	58.2	58.3	458-494 bp	1.0	80.6
trnG-GCC-trnfM-CAU_p4	ATATAGGACGTAGCGAGCGT	CAGTTTGGTAGCTCACGAGG	50.0	55.0	58.2	58.3	466-502 bp	1.0	80.5
trnG-GCC-trnfM-CAU_p5	CGTAGCGAGCGTAGTTCAAT	TACAGAGCGGAGTAGAGCAG	50.0	55.0	58.2	58.3	475-511 bp	1.0	80.3