Hotspot

trnS-GCU-psbD

LSC · 8107-9102 · 996 bp

Back to Dichanthelium

Pi 0.00261

MarkerSeek score 61.80

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 996
variable_sites: 7
pi: 0.00261
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1118-1142 bp	1.0	81.4
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1105-1129 bp	1.0	80.8
trnS-GCU-psbD_p3	GCTTTAGTCCACTCAGCCAT	GCCGGACCATCCTACAAAAA	50.0	50.0	57.6	58.2	1104-1128 bp	1.0	78.4
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	GAAACGGTCCCTTCGTAACC	45.0	55.0	58.0	58.6	1097-1121 bp	1.0	78.2
trnS-GCU-psbD_p5	GCTTTAGTCCACTCAGCCAT	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.6	58.2	1091-1115 bp	1.0	77.9