Hotspot

trnP-UGG-psaJ

LSC · 69394-69766 · 373 bp

Back to Diarthron

Pi 0.0539

MarkerSeek score 70.80

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 373
variable_sites: 20
pi: 0.0539
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	GGAAAGATATGTTTTTAGATCTCGCA	45.0	34.6	57.9	57.9	435-437 bp	1.0	44.0
trnP-UGG-psaJ_p2	CAAAACAAACGCGCTACCAA	GGAAAGATATGTTTTTAGATCTCGCA	45.0	34.6	58.2	57.9	436-438 bp	1.0	44.0
trnP-UGG-psaJ_p3	TTGTACCCAAAACAAACGCG	GGAAAGATATGTTTTTAGATCTCGCA	45.0	34.6	57.8	57.9	443-445 bp	1.0	44.0
trnP-UGG-psaJ_p4	TACCCAAAACAAACGCGCTA	GGAAAGATATGTTTTTAGATCTCGCA	45.0	34.6	58.4	57.9	440-442 bp	1.0	44.0
trnP-UGG-psaJ_p5	AAAACAAACGCGCTACCAAG	GGAAAGATATGTTTTTAGATCTCGC	45.0	36.0	57.9	56.4	435-437 bp	1.0	44.0