Hotspot

psbE-petL

LSC · 67309-68504 · 1196 bp

Back to Diarthron

Pi 0.0293

MarkerSeek score 69.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1196
variable_sites: 35
pi: 0.0293
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCTGAGGTTATAGTTAAAGCCA	50.0	39.1	57.9	57.3	1360-1582 bp	1.0	52.0
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGTCGTATCTTACTCAGACCA	50.0	42.9	57.9	55.3	1389-1611 bp	1.0	51.8
psbE-petL_p3	CAACCCGCAATGAATAGGGA	GCTGAGGTTATAGTTAAAGCCAC	50.0	43.5	57.9	57.3	1359-1581 bp	1.0	51.7
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGCTGAGGTTATAGTTAAAGCCA	50.0	39.1	57.8	57.3	1283-1505 bp	1.0	51.3
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGCTGAGGTTATAGTTAAAGCCA	45.0	39.1	58.2	57.3	1369-1591 bp	1.0	51.0