Hotspot

trnL-UAA-trnF-GAA

LSC · 50848-51225 · 378 bp

Back to Diarthron

Pi 0.0511

MarkerSeek score 71.80

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 378
variable_sites: 19
pi: 0.0511
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	462-468 bp	1.0	80.1
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGA	50.0	50.0	57.5	57.6	424-430 bp	1.0	77.3
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	459-465 bp	1.0	76.9
trnL-UAA-trnF-GAA_p4	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	451-457 bp	1.0	76.3
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TGCCGAGAACCAGATTTGAA	50.0	45.0	57.5	57.4	476-482 bp	1.0	75.9