Hotspot

rps14-psaB

LSC · 39543-39668 · 126 bp

Back to Diarthron

Pi 0.0476

MarkerSeek score 70.20

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 126
variable_sites: 6
pi: 0.0476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	TGCCTCTTCTTCTCCCTATGA	CCAGTGGCTCTTTCCATTGT	47.6	50.0	57.6	58.1	284 bp	1.0	69.8
rps14-psaB_p2	TGCCTCTTCTTCTCCCTATGA	GCCTCTACATCGGGCAAATT	47.6	50.0	57.6	58.3	194 bp	1.0	68.7
rps14-psaB_p3	TGCCTCTTCTTCTCCCTATGA	GCTCTTTCCATTGTGCAAGC	47.6	50.0	57.6	58.3	278 bp	1.0	68.1
rps14-psaB_p4	TGCCTCTTCTTCTCCCTATGA	TGGCTCTTTCCATTGTGCAA	47.6	45.0	57.6	58.3	280 bp	1.0	68.1
rps14-psaB_p5	TTTTGCCTCTTCTTCTCCCT	CCAGTGGCTCTTTCCATTGT	45.0	50.0	56.3	58.1	287 bp	1.0	67.8