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psbZ-trnG-GCC

LSC · 38196-38785 · 590 bp

Back to Diarthron

Pi 0.0281

MarkerSeek score 73.20

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 16
pi: 0.0281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	AATCTATTACCCTGGGCGGA	50.0	50.0	57.8	57.9	785-800 bp	1.0	81.1
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTT	AATCTATTACCCTGGGCGGA	50.0	50.0	57.8	57.9	783-798 bp	1.0	81.1
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	747-762 bp	1.0	80.8
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	745-760 bp	1.0	80.8
psbZ-trnG-GCC_p5	TTGCTTCTCCTGATGGTTGG	ATCTATTACCCTGGGCGGAT	50.0	50.0	57.8	57.7	784-799 bp	1.0	79.2