Hotspot

trnT-GGU-psbD

LSC · 33613-34849 · 1237 bp

Back to Diarthron

Pi 0.0297

MarkerSeek score 68.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1237
variable_sites: 30
pi: 0.0297
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1178-1379 bp	1.0	85.3
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1181-1382 bp	1.0	85.2
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1155-1356 bp	1.0	83.6
trnT-GGU-psbD_p4	TGGCCCTTTTAACTCAGTGG	GAAACGGTCCCTACGTAACC	50.0	55.0	57.7	58.0	1194-1395 bp	1.0	83.5
trnT-GGU-psbD_p5	GGCCCTTTTAACTCAGTGGT	GAAACGGTCCCTACGTAACC	50.0	55.0	57.7	58.0	1193-1394 bp	1.0	83.5