Hotspot

psbM-trnD-GUC

LSC · 31500-32247 · 748 bp

Back to Diarthron

Pi 0.043

MarkerSeek score 77.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 32
pi: 0.043
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATCAACAGCGCAGTAGCAAT	AGTTCAACTGGTCAGAGCAC	45.0	50.0	58.3	57.8	858-862 bp	1.0	79.2
psbM-trnD-GUC_p2	AATTGCCTTGGCTGACTGTT	AGTTCAACTGGTCAGAGCAC	45.0	50.0	58.3	57.8	917-921 bp	1.0	78.7
psbM-trnD-GUC_p3	ATTGCCTTGGCTGACTGTTT	AGTTCAACTGGTCAGAGCAC	45.0	50.0	58.3	57.8	916-920 bp	1.0	78.7
psbM-trnD-GUC_p4	TCAACAGCGCAGTAGCAATA	AGTTCAACTGGTCAGAGCAC	45.0	50.0	57.6	57.8	857-861 bp	1.0	78.3
psbM-trnD-GUC_p5	AATCAACAGCGCAGTAGCAA	AGTTCAACTGGTCAGAGCAC	45.0	50.0	58.5	57.8	859-863 bp	1.0	77.5