Hotspot

petN-psbM

LSC · 30325-31394 · 1070 bp

Back to Diarthron

Pi 0.0314

MarkerSeek score 66.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1070
variable_sites: 23
pi: 0.0314
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	ATTGCTACTGCGCTGTTGAT	45.0	45.0	58.0	58.3	911-1227 bp	1.0	83.6
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	AAACAGTCAGCCAAGGCAAT	45.0	45.0	58.0	58.3	853-1169 bp	1.0	83.4
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	AACAGTCAGCCAAGGCAATT	45.0	45.0	58.0	58.3	852-1168 bp	1.0	83.4
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TATTGCTACTGCGCTGTTGA	45.0	45.0	58.0	57.6	912-1228 bp	1.0	82.6
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	ATTGCTACTGCGCTGTTGAT	50.0	45.0	57.8	58.3	917-1233 bp	1.0	82.1