Hotspot

rps15

SSC · 117352-117621 · 270 bp

Back to Dianella

Pi 0.00741

MarkerSeek score 49.60

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 270
variable_sites: 2
pi: 0.00741
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	CTGATCGGTAAAATCCACGGA	TCAAAACGAGATTCATCAAAACGA	47.6	33.3	57.8	57.7	412 bp	1.0	40.4
rps15_p2	TCCTGATCGGTAAAATCCACG	TCAAAACGAGATTCATCAAAACGA	47.6	33.3	57.8	57.7	414 bp	1.0	40.4
rps15_p3	CTGATCGGTAAAATCCACGGA	ACGAGATTCATCAAAACGAAAAGT	47.6	33.3	57.8	57.7	407 bp	1.0	40.4
rps15_p4	TCCTGATCGGTAAAATCCACG	ACGAGATTCATCAAAACGAAAAGT	47.6	33.3	57.8	57.7	409 bp	1.0	40.4
rps15_p5	TGATCGGTAAAATCCACGGA	TCAAAACGAGATTCATCAAAACGA	45.0	33.3	56.6	57.7	411 bp	1.0	40.4