Hotspot

infA-rps8

LSC · 81672-81815 · 144 bp

Back to Dianella

Pi 0.0139

MarkerSeek score 60.50

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 144
variable_sites: 2
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	GACCTGGAACATACGGTTGG	CTTTCCACTTCTCGAGGCAT	55.0	50.0	58.3	57.6	277 bp	1.0	75.3
infA-rps8_p2	GGACCTGGAACATACGGTTG	CTTTCCACTTCTCGAGGCAT	55.0	50.0	58.3	57.6	278 bp	1.0	75.3
infA-rps8_p3	GACCTGGAACATACGGTTGG	TTCTTTCCACTTCTCGAGGC	55.0	50.0	58.3	57.5	279 bp	1.0	74.8
infA-rps8_p4	GGACCTGGAACATACGGTTG	TTCTTTCCACTTCTCGAGGC	55.0	50.0	58.3	57.5	280 bp	1.0	74.8
infA-rps8_p5	TACGGTTGGGAAGTGATTCG	CTTTCCACTTCTCGAGGCAT	50.0	50.0	57.6	57.6	266 bp	1.0	74.5