Hotspot

trnF-ndhJ

LSC · 49275-49755 · 481 bp

Back to Dianella

Pi 0.0146

MarkerSeek score 59.80

Primer available yes

Label trnF-ndhJ

Feature Metadata

label_name: trnF-ndhJ
feature_type: igs
region: LSC
length_bp: 481
variable_sites: 7
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	591-593 bp	1.0	79.5
trnF-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	581-583 bp	1.0	78.9
trnF-ndhJ_p3	ATGATGCGCGAAAAATGGTC	TGAAAGTTGGATAGGCTGGC	45.0	50.0	57.8	57.9	647-649 bp	1.0	78.3
trnF-ndhJ_p4	GATGATGCGCGAAAAATGGT	TGAAAGTTGGATAGGCTGGC	45.0	50.0	57.8	57.9	648-650 bp	1.0	78.3
trnF-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	633-635 bp	1.0	78.2