Hotspot

ycf1-ndhF

SSC · 114092-114096 · 5 bp

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Pi 0.267

MarkerSeek score 60.90

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 5
variable_sites: 2
pi: 0.267
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	AAGCACAAAAGAGAGCCACA	GTAGGGGGTGGTCGAATTTC	45.0	55.0	57.9	58.0	526-587 bp	1.0	80.1
ycf1-ndhF_p2	ACAAGCACAAAAGAGAGCCA	GTAGGGGGTGGTCGAATTTC	45.0	55.0	57.9	58.0	528-589 bp	1.0	80.1
ycf1-ndhF_p3	AAGCACAAAAGAGAGCCACA	TAGGGGGTGGTCGAATTTCT	45.0	50.0	57.9	58.0	525-586 bp	1.0	80.1
ycf1-ndhF_p4	ACAAGCACAAAAGAGAGCCA	TAGGGGGTGGTCGAATTTCT	45.0	50.0	57.9	58.0	527-588 bp	1.0	80.1
ycf1-ndhF_p5	AAGCACAAAAGAGAGCCACA	GAATTCCAAATGGGGTCGGT	45.0	50.0	57.9	58.2	582-643 bp	1.0	79.1