Hotspot

rpl33-rps18

LSC · 70710-71053 · 344 bp

Back to Dialium

Pi 0.0407

MarkerSeek score 61.60

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 344
variable_sites: 21
pi: 0.0407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	GCAAACATAGGATTCACGCG	GCAAACGCCTACGAAAAGAT	50.0	45.0	57.9	57.1	399-437 bp	1.0	80.1
rpl33-rps18_p2	TCTGTCCCTGTTGTTGCAAA	GCAAACGCCTACGAAAAGAT	45.0	45.0	57.9	57.1	414-452 bp	1.0	79.6
rpl33-rps18_p3	TTCTGTCCCTGTTGTTGCAA	GCAAACGCCTACGAAAAGAT	45.0	45.0	57.9	57.1	415-453 bp	1.0	79.6
rpl33-rps18_p4	AACATAGGATTCACGCGGAG	GCAAACGCCTACGAAAAGAT	50.0	45.0	57.8	57.1	396-434 bp	1.0	79.0
rpl33-rps18_p5	GCAAACATAGGATTCACGCG	GGCAAACGCCTACGAAAAGA	50.0	50.0	57.9	59.1	400-438 bp	1.0	78.2